- Facility
Biophysics & Structural Biology (B2S)
- Microcalorimetry, Circular Dichroïsm, Surface Plasmon Resonance, NMR, Chromatography, Light Scattering, Fast kinetics, Cryomicroscopy
The Biophysics and Structural Biology (B2S) platform offers a combination of equipment for in vitro physicochemical characterization of proteins and interactions. It provides access to several biophysical (circular dichroism, microcalorimetry, surface plasmon resonance, fluorescence) and structural biology (NMR, X-ray) devices, and is based on the skills of the IMoPA research teams. It belongs to the ARBRE association (Association of Resources for Biophysical Research in Europe) created in 2015. It allows the development of multidisciplinary projects to characterize 1) the interactions involved between biological macromolecules and, 2) the 3D structure biological macromolecules.
The B2S staff has a real technical and methodological expertise to address different issues: The stability / characterization of proteins and complexes in solution, the three-dimensional structure of proteins and complexes, fast kinetics, the characterization of protein-protein and protein-ligand interactions as well as the isolation and characterization of a broad spectrum of products by liquid chromatography.
The platform operates in two modes: 1) the service mode (turnkey services along with, or not, collaborations) and 2) the provision of devices for expert users after initial training.
Any new collaborator must contact the person in charge of the Core Facility to define the best strategy to adopt for the project and complete the service request form. Any user will accept the terms and conditions for service use by signing the agreement form.
We are always available for users during the use of the devices and for any advice and assistance.
Each user must retrieve its data at the end of the analyses. In the usual case, the raw acquisition data are kept for 1 year on computer stations. The platform does not guarantee the recovery of data in case of malfunction. The user is responsible for the final data storage.
- Mathieu J, Kriznik A, Charron C, Perchat-Varlet R, Selles B, Rahuel-Clermont S. The Dual Role of Active Site Hydroxylated Residue in Peroxiredoxin Sulfinylation Catalysis. Antioxid Redox Signal. 2025 Jul;43(1-3):1-13.
10.1089/ars.2024.0685 , 
40066651 , 
HAL-05215148 - Guiot E, Chagot ME, Boutilliat A, Reboul P, Kriznik A, Quinternet M. CRDSAT under Heat: Balancing Stability, Affinity, and Functional Utility of Computationally Designed Tag Variants. Biochemistry. 2025 Sep 2;64(17):3688-3694.
10.1021/acs.biochem.5c00128 ,
40610384
Faure R, Kapel R, Marchal P, Génin G, Beaubier S. Enzymatic crosslinking of rapeseed albumins: kinetic monitoring, physical characteristics, and functional property enhancements. Food Hydrocolloids, Volume 172, Part 2, 2025.
10.1016/j.foodhyd.2025.112100 - Lec JC, Claudel AL, Boutserin S, Mazon H, Mulliert G, Boschi-Muller S, Talfournier F. The Catalytic Promiscuity of TSTD1-Like Sulfurtransferases Originates from a Bifaceted Active Site. ACS Catalysis, 2025, 15 (21), pp.18535-18543.
10.1021/acscatal.5c05390
- Scat S, Weissman KJ, Chagot B. Insights into docking in megasynthases from the investigation of the toblerol trans-AT polyketide synthase: many α-helical means to an end. RSC Chem Biol. 2024 May 16;5(7):669-683.
10.1039/d4cb00075g ,
38966669
Collin S, Cox RJ, Paris C, Jacob C, Chagot B, Weissman KJ, Gruez A. Decrypting the programming of β-methylation in virginiamycin M biosynthesis. Nat Commun. 2023 Mar 10;14(1):1327.
10.1038/s41467-023-36974-3 ,
36899003 Hoschtettler P, Pickaert G, Carvalho A, Averlant-Petit MC, Stefan L. Highly Synergistic Properties of Multicomponent Hydrogels Thanks to Cooperative Nucleopeptide Assemblies. Chemistry of Materials. 2023 May 17.
10.1021/acs.chemmater.3c00308 - Dos Santos Morais R, Santo PE, Ley M, Schelcher C, Abel Y, Plassart L, Deslignière E, Chagot ME, Quinternet M, Paiva ACF, Hessmann S, Morellet N, M F Sousa P, Vandermoere F, Bertrand E, Charpentier B, Bandeiras TM, Plisson-Chastang C, Verheggen C, Cianférani S, Manival X. Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases. J Mol Biol. 2022 Jul 25.
10.1016/j.jmb.2022.167760 ,
35901867 ,
HAL-03745381
Chagot ME, Boutilliat A, Kriznik A, Quinternet M. Structural Analysis of the Plasmodial Proteins ZNHIT3 and NUFIP1 Provides Insights into the Selectivity of a Conserved Interaction. Biochemistry. 2022 Mar 22.
10.1021/acs.biochem.1c00792 ,
35315277 ,
HAL-03617333 Beaussart A, Canonico F, Mazon H, Hidalgo J, Cianférani S, Le Cordier H, Kriznik A, Rahuel-Clermont S. Probing the mechanism of the peroxiredoxin decamer interaction with its reductase sulfiredoxin from the single molecule to the solution scale. Nanoscale Horiz. 2022 Mar 2.
10.1039/d2nh00037g ,
35234779 ,
HAL-03595966
