The proteomics Core Facility assists researchers in protein identification, quantification and chemical modification characterization.
We are specialized in label-free relative quantifcation and in developping financially affordable analytical methods, in order to promote the use of proteomics tools in all fields of biology. We now also offer the quantification of animo acids, either free or hydrolyzed from proteins.
We develop specific sample preparation methods, in particular in the field of extracellular matrix biology.
The Core Facility is open to the academic and industrial scientific community at the local, national and international level.
The Core Facility's equipment is not directly accessible to users.
The Core Facility operates as a service delivery system, with pricing adapted to the type of service and institutional origin of the users and requires compliance to terms and conditions.
For any request, contact the facility manager with a brief description of your study. The discussion may lead to a proposal for a quotation, which must be validated by the user before carrying out the analyses.
Wilhelm D, Wurtz A, Abouelfarah H, Sanchez G, Bui C, Vincourt JB. Tissue-specific collagen hydroxylation at GEP/GDP triplets mediated by P4HA2. Matrix Biol. 2023 Mar 30:S0945-053X(23)00046-X.
10.1016/j.matbio.2023.03.009 , 
37003347 , 
HAL-04077010
10.3389/fbioe.2022.884069 ,
35769101 ,
HAL-03753092 Wilhelm D, Kempf H, Bianchi A, Vincourt JB. ATDC5 cells as a model of cartilage extracellular matrix neosynthesis, maturation and assembly. J Proteomics. 2020 May 15 ; 219:103718.
10.1016/j.jprot.2020.103718 ,
32097723 ,
HAL-02938632 Nguyen DV, Roret T, Fernandez-Gonzalez A, Kohler A, Morel-Rouhier M, Gelhaye E, Sormani R. Target Of Rapamycin pathway in the white-rot fungus Phanerochaete chrysosporium. PLoS One. 2020 Feb 20 ; 15(2):e0224776.
10.1371/journal.pone.0224776 ,
32078630 Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ. Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: Docking in the enacyloxin IIa polyketide synthase. J Struct Biol. 2020 Oct 1 ; 212(1):107581.
10.1016/j.jsb.2020.107581 ,
32717326 Henrionnet C, Gillet P, Mainard D, Vincourt JB, Pinzano A. Label-free relative quantification of secreted proteins as a non-invasive method for the quality control of chondrogenesis in bioengineered substitutes for cartilage repair. J Tissue Eng Regen Med. 2018 Mar ; 12(3):e1757-e1766.
10.1002/term.2454 ,
28485490 ,
HAL-01745215 Yang X, Qi Y, Avercenc-Leger L, Vincourt JB, Hupont S, Huselstein C, Wang H, Chen L, Magdalou J. Effect of nicotine on the proliferation and chondrogenic differentiation of the human Wharton's jelly mesenchymal stem cells. Biomed Mater Eng. 2017 ; 28(s1):S217-S228.
10.3233/BME-171644 ,
28372298 ,
HAL-01708965 Belcastro E, Wu W, Fries-Raeth I, Corti A, Pompella A, Leroy P, Lartaud I, Gaucher C. Oxidative stress enhances and modulates protein S-nitrosation in smooth muscle cells exposed to S-nitrosoglutathione. Nitric Oxide. 2017 Sep 30 ; 69:10-21.
10.1016/j.niox.2017.07.004 ,
28743484 
The proteomics platform offers two training sessions (12 March 2019 and 24 September 2019), on the principles and methodologies of protein analysis in complex mixtures by unlabelled mass spectrometry, with practical training.
To participate, please send an email to jean-baptiste [mfundo] vincourt [chizindikiro] univ-lorraine [mfundo] fr.
For more information: http://ibslor-proteomics.cnrs.fr/lc-maldi-training/
