- Facility
Flow Cytometry
, 
- Cell cycle, Extracellular vesicles, Phenotyping, Sorting, Apoptosis, Mitochondrial respiration and glycolysis
The Flow Cytometry-Cell Biology Core Facility is an integral part of the UMS2008/US40 Engineering Biology Health in Lorraine.
Located on the Brabois-Santé campus of the University of Lorraine in Nancy, it offers innovative equipment for all applications dealing with cell analysis and cell sorting. It offers three types of services with an appropriate environment to guarantee support for the development of scientific projects:
- Flow cytometry:
- Flow cytometry analyzer Gallios, Beckman Coulter: 3 lasers - 10 colours : Training, availability of the instrument and realization of analysis by the staff of the Core Facility.
- Flow cytometry analyzer Celesta Sorp, BD Biosciences: 4 lasers - 16 colours : Training, availability of the instrument and realization of analysis by the staff of the Core Facility.
- Flow cytometry analyzer Symphony A3, BD Biosciences: 5 lasers - 28 colours : Training, availability of the instrument and realization of analysis by the staff of the Core Facility.
- Flow analysis cell - Sorter Astrios, Beckman Coulter: 3 lasers - 14 colours : Realization of sorting by the staff of the Core Facility.
- Seahorse XFp Analyzer Cell Metabolism: Training and availability of the instrument.
The platform provides expertise and technical assistance in order to support research projects. The Core Facility puts its expertise at the service of users in various fields (cell cycle analysis and apoptosis, rare cell sorting, multiparametric sorting). It has recognized technical know-how in the detection and standardization in the analysis of circulating human microparticles. The main objective is to satisfy all requests in flow cytometry and analysis related to laboratory research, and to be open to any academic scientific (CNRS, INSERM, UL ... ) and industrial community.
Our missions are to adapt the methodological developments of the cytometry Core Facility in relation with users' projects, to propose activities of transfer of knowledge of the domain to scientists, students and staff in training courses. We maintain a technology watch associated with the increase in the competence of the service offered.
The staff works to ensure the good functioning of the platform:
- Organization of the activity (reservation planning, data storage).
- Training / user support.
- Project monitoring and analysis.
- Laintenance and management of hardware and equipment.
Since 2020, it has been awarded the Research Support Structure (StAR-LUE) label as part of the INFRA + program of LUE (Lorraine University of Excellence).
The Core Facility has a differential pricing system (UMS member teams, academic teams, and industrial teams)
- Flow cytometry:
- Request form - Flow cytometry equipment208.46 KBRequest form for availability - Flow cytometry equipment202.53 KBRequest form - Astrios244.65 KBTerms and conditions238.54 KB
The Core Facility is open to the academic and industrial scientific community at the local, national and international level.
The team is at your disposal to help you by bringing its technical and methodological know-how and to follow you from A to Z in your cytometry projects.
The Core Facility has a differential pricing system (members, academic and industrial).
Any new collaborator must first contact the head of the core facility using the request form to define the best strategy to adopt for conducting his study, and obtain a quote.
Any user will agree to accept the terms and conditions.
To book an equipment, click here.
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Flow Cytometry Training - Autonomy on Celesta and Symphony
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The Flow Cytometry Core Facility offers two training sessions (February and September-October) "Cytometry-Celesta and Symphony".
Program of a training course :
Theoretical (2h):
- Theoretical foundations of flow cytometry. Principles of operation.
- Reminder of some immunotechnology concepts.
- Multiparametric analysis: interest and associated constraints.
Practical (4h):
- Take charge of the instrument. Conditions of use. Discovery of the acquisition software.
- Creation of protocols and protocol suites.
- Instrument settings. Introduction of a multicolour analysis.
- Exploitation of the reprocessing capabilities of the acquisition software.
Trieur Astrios & Cytomètre Symphony :
Service Mutualisé de Plateformes
Biopôle, Campus Brabois-Santé
9 Avenue de la Forêt de Haye, BP 20199
54505 VANDOEUVRE-LES-NANCYCytomètres Celesta & Gallios :
Service Mutualisé de Plateformes - UMR1116 DCAC
Bâtiment D, 1er étage, Campus Brabois-Santé
9 Avenue de la Forêt de Haye, BP 20199
54505 VANDOEUVRE-LES-NANCY
