- Facility
Biophysics & Structural Biology (B2S)
- Microcalorimetry, Circular Dichroïsm, Surface Plasmon Resonance, NMR, Chromatography, Light Scattering, Fast kinetics, Cryomicroscopy
The Biophysics and Structural Biology (B2S) platform offers a combination of equipment for in vitro physicochemical characterization of proteins and interactions. It provides access to several biophysical (circular dichroism, microcalorimetry, surface plasmon resonance, fluorescence) and structural biology (NMR, X-ray) devices, and is based on the skills of the IMoPA research teams. It belongs to the ARBRE association (Association of Resources for Biophysical Research in Europe) created in 2015. It allows the development of multidisciplinary projects to characterize 1) the interactions involved between biological macromolecules and, 2) the 3D structure biological macromolecules.
The B2S staff has a real technical and methodological expertise to address different issues: The stability / characterization of proteins and complexes in solution, the three-dimensional structure of proteins and complexes, fast kinetics, the characterization of protein-protein and protein-ligand interactions as well as the isolation and characterization of a broad spectrum of products by liquid chromatography.
The platform operates in two modes: 1) the service mode (turnkey services along with, or not, collaborations) and 2) the provision of devices for expert users after initial training.
Any new collaborator must contact the person in charge of the Core Facility to define the best strategy to adopt for the project and complete the service request form. Any user will accept the terms and conditions for service use by signing the agreement form.
We are always available for users during the use of the devices and for any advice and assistance.
Each user must retrieve its data at the end of the analyses. In the usual case, the raw acquisition data are kept for 1 year on computer stations. The platform does not guarantee the recovery of data in case of malfunction. The user is responsible for the final data storage.
- Abel Y, Charron C, Virciglio C, Bourguignon-Igel V, Quinternet M, Chagot ME, Robert MC, Verheggen C, Branlant C, Bertrand E, Manival X, Charpentier B, Rederstorff M. The interaction between RPAP3 and TRBP reveals a possible involvement of the HSP90/R2TP chaperone complex in the regulation of miRNA activity. Nucleic Acids Res. 2022 Feb 28;50(4):2172-2189.
10.1093/nar/gkac086 , 
35150569 , 
HAL-03583324 Motorin Y, Quinternet M, Rhalloussi W, Marchand V. Constitutive and variable 2'-O-methylation (Nm) in human ribosomal RNA. RNA Biol. 2021 Sep 10:1-10.
10.1080/15476286.2021.1974750 ,
34503375 ,
HAL-03341858 - Bragantini B, Charron C, Bourguet M, Paul A, Tiotiu D, Rothé B, Marty H, Terral G, Hessmann S, Decourty L, Chagot ME, Strub JM, Massenet S, Bertrand E, Quinternet M, Saveanu C, Cianférani S, Labialle S, Manival X, Charpentier B. The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity. Nat Commun. 2021 Mar 25;12(1):1859.
10.1038/s41467-021-22077-4 ,
33767140 ,
HAL-03181046
Dermouche S, Chagot ME, Manival X, Quinternet M. Optimizing the First TPR Domain of the Human SPAG1 Protein Provides Insight into the HSP70 and HSP90 Binding Properties. Biochemistry. 2021 Mar 19.
10.1021/acs.biochem.1c00052 ,
33739091 ,
HAL-03175986 - Chagot ME, Quinternet M, Manival X, Lebars I. Application of NMR Spectroscopy to Determine the 3D Structure of Small Non-Coding RNAs. Methods Mol Biol. 2021;2300:251-266.
10.1007/978-1-0716-1386-3_19 ,
33792884 ,
HAL-03203667
Kriznik A, Libiad M, Le Cordier H, Boukhenouna S, Toledano MB, Rahuel-Clermont S. Dynamics of a key conformational transition in the mechanism of peroxiredoxin sulfinylation. ACS Catalysis. 2020.
10.1021/acscatal.9b04471 ,
HAL-02473398 Chagot ME, Quinternet M, Jacquemin C, Manival X, Gardiennet C. Box C/D snoRNPs: solid-state NMR fingerprint of an early-stage 50 kDa assembly intermediate. Biomol NMR Assign. 2020 Feb 6.
10.1007/s12104-020-09933-y ,
32030621 ,
HAL-02480749 Dreyer A, Schackmann A, Kriznik A, Chibani K, Wesemann C, Vogelsang L, Beyer A, Dietz KJ. Thiol Redox Regulation of Plant β-Carbonic Anhydrase. Biomolecules. 2020 Jul 30.
10.3390/biom10081125 ,
32751472 ,
HAL-02917419 Albe Slabi S, Mathe C, Basselin M, Fournier F, Aymes A, Bianeis M, Galet O, Kapel R. Optimization of sunflower albumin extraction from oleaginous meal and characterization of their structure and properties. Food Hydrocolloids, Volume 99, 2020 Feb, 105335.
10.1016/j.foodhyd.2019.105335 - Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ. Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. J Struct Biol. 2020 Jul 25.
10.1016/j.jsb.2020.107581 ,
32717326
