Facility
Biophysics & Structural Biology (B2S)
ITC
Microcalorimetry allows the direct and precise thermodynamic characterization of interactions between molecules in solution and biochemical reactions in general. Isothermal calorimeters (VP-ITC and ITC200, MicroCal) are mainly dedicated to the study of molecular interactions. Isothermal calorimetry (ITC) is the only real direct method for measuring an association constant in solution, without the need to label molecules. ITC is relatively unaffected by artifacts and is completely independent of the optical properties of the samples, or the size of the ligand or protein. A direct measurement using the ITC200 makes possible to detect affinities between nanomolar and millimolar, whereas measurements by competition with a ligand of known affinity allows to evaluate affinities of the picomolar order.
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Analyzes can be performed in service mode or device provision mode after initial training. Contact the person in charge using the Request form for any information.
- Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ. Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. J Struct Biol. 2020 Jul 25.
10.1016/j.jsb.2020.107581 , 
32717326 - Dos Santos Morais R, Santo PE, Ley M, Schelcher C, Abel Y, Plassart L, Deslignière E, Chagot ME, Quinternet M, Paiva ACF, Hessmann S, Morellet N, M F Sousa P, Vandermoere F, Bertrand E, Charpentier B, Bandeiras TM, Plisson-Chastang C, Verheggen C, Cianférani S, Manival X. Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases. J Mol Biol. 2022 Jul 25.
10.1016/j.jmb.2022.167760 ,
35901867 ,
HAL-03745381 Chagot ME, Boutilliat A, Kriznik A, Quinternet M. Structural Analysis of the Plasmodial Proteins ZNHIT3 and NUFIP1 Provides Insights into the Selectivity of a Conserved Interaction. Biochemistry. 2022 Mar 22.
10.1021/acs.biochem.1c00792 ,
35315277 ,
HAL-03617333 Dermouche S, Chagot ME, Manival X, Quinternet M. Optimizing the First TPR Domain of the Human SPAG1 Protein Provides Insight into the HSP70 and HSP90 Binding Properties. Biochemistry. 2021 Mar 19.
10.1021/acs.biochem.1c00052 ,
33739091 ,
HAL-03175986 Chagot ME, Dos Santos Morais R, Dermouche S, Lefebvre D, Manival X, Chipot C, Dehez F, Quinternet M. Binding properties of the quaternary assembly protein SPAG1. Biochem J. 2019 May 22.
10.1042/BCJ20190198 ,
31118266 ,
HAL-02140524 Chagot ME, Quinternet M, Rothé B, Charpentier B, Coutant J, Manival X, Lebars I. The yeast C/D box snoRNA U14 adopts a "weak" K-turn like conformation recognized by the Snu13 core protein in solution. Biochimie. 2019 Mar 23.
10.1016/j.biochi.2019.03.014 ,
30914254 ,
HAL-02082477 Carrasco K, Boufenzer A, Jolly L, Le Cordier H, Wang G, Heck AJ, Cerwenka A, Vinolo E, Nazabal A, Kriznik A, Launay P, Gibot S, Derive M. TREM-1 multimerization is essential for its activation on monocytes and neutrophils. Cell Mol Immunol. 2018 Mar 22.
10.1038/s41423-018-0003-5 ,
29568119 ,
HAL-01945617 Bersweiler A, D'Autréaux B, Mazon H, Kriznik A, Belli G, Delaunay-Moisan A, Toledano MB, Rahuel-Clermont S. A scaffold protein that chaperones a cysteine-sulfenic acid in H(2)O(2) signaling. Nat Chem Biol. 2017 Aug ; 13(8):909-915.
10.1038/nchembio.2412 ,
28628095 ,
HAL-01652643 Maurizy C, Quinternet M, Abel Y, Verheggen C, Santo PE, Bourguet M, Paiva A, Bragantini B, Chagot ME, Robert MC, Abeza C, Fabre P, Fort P, Vandermoere F,Sousa P Rain JC, Charpentier B, Cianférani S, Bandeiras TM, Pradet-Balade B, Manival X, Bertrand E. The RPAP3-Cterminal domain identifies R2TP-like quaternary chaperones. Nat Commun. 2018 May 29 ; 9(1):2093.
10.1038/s41467-018-04431-1 ,
29844425 ,
HAL-01872981 Henri J, Chagot ME, Bourguet M, Abel Y, Terral G, Maurizy C, Aigueperse C, Georgescauld F, Vandermoere F, Saint-Fort R, Behm-Ansmant I, Charpentier B, Pradet-Balade B, Verheggen C, Bertrand E, Meyer P, Cianférani S, Manival X, Quinternet M. Deep Structural Analysis of RPAP3 and PIH1D1, Two Components of the HSP90 Co-chaperone R2TP Complex. Structure. 2018 Sep 4 ; 26(9):1196-1209.
10.1016/j.str.2018.06.002 ,
30033218 ,
HAL-01919243 Kriznik A, Yéléhé-Okouma M, Lec JC, Groshenry G, Le Cordier H, Charron C, Quinternet M, Mazon H, Talfournier F, Boschi-Muller S, Jouzeau JY, Reboul P. CRDSAT Generated by pCARGHO: A New Efficient Lectin-Based Affinity Tag Method for Safe, Simple, and Low-Cost Protein Purification. Biotechnol J. 2018 Oct 8:e1800214.
10.1002/biot.201800214 ,
30298550 ,
HAL-01892441
