- Facility
Epitranscriptomics & Sequencing (EpiRNA-Seq)
- Next-Generation Sequencing, RNA, RNA modifications
The Epitranscriptomics and Sequencing (EpiRNA-Seq) Core Facility is built on the expertise of the UMR7365 IMoPA in the field of RNA molecular biology to promote its main collaborative services.
Located on the Brabois-Santé campus of the University of Lorraine in Nancy, it provides to the academic and industrial scientific community high-tech resources (Illumina next generation sequencers). It is part of the European COST EPITRAN network and regularly organises training activities.
The core facility has recognized technical and methodological know-how to contribute to various projects in Epitranscriptomics (RiboMethSeq, AlkAniline-Seq, HydraPsiSeq) to address various scientific questions such as dynamics of post-transcriptional modifications in RNAs.
The EpiRNA-Seq Core Facility operates mainly in collaborative mode (involvement of members of the core facility in the steps of your sequencing project from the conception of the experimental design to the statistical analysis of the data) but the delivery mode (mainly sequencing of libraries prepared by you without involvement of the platform) is also possible under certain conditions. The EpiRNA-Seq Core Facility has a differential pricing system (Université de Lorraine members, academics and industrialists).
The EpiRNA-Seq Core Facility is open to the academic and industrial scientific community at the local, national and international level.
The team is at your disposal to help you by bringing its technical and methodological know-how and to follow you from A to Z in your sequencing projects.
The EpiRNA-Seq Core Facility has a differential pricing system (Université de Lorraine members, academic and industrial).
Any new collaborator must first contact the head of the core facility using the "Request form" to define the best strategy to adopt for conducting his study, and obtain a quote. Any user will agree to accept the "terms and conditions". The guidelines to prepare and send samples to the Core Facility are given in "Sample sheet".
Freund I, Buhl DK, Boutin S, Kotter A, Pichot F, Marchand V, Vierbuchen T, Heine H, Motorin Y, Helm M, Dalpke AH, Eigenbrod T. 2'-O-methylation within prokaryotic and eukaryotic tRNA inhibits innate immune activation by endosomal Toll-like receptors but does not affect recognition of whole organisms. RNA. 2019 Apr 24.
10.1261/rna.070243.118 , 
31019095 , 
HAL-02113259 Jacob D, Thüring K, Galliot A, Marchand V, Galvanin A, Ciftci A, Scharmann K, Stock M, Roignant JY, Leidel SA, Motorin Y, Schaffrath R, Klassen R, Helm M. Absolute quantification of noncoding RNA by microscale thermophoresis. Angew Chem Int Ed Engl. 2019 Mar 20.
10.1002/anie.201814377 ,
30892798 ,
HAL-02078064 Wei J, Kishton RJ, Angel M, Conn CS, Dalla-Venezia N, Marcel V, Vincent A, Catez F, Ferré S, Ayadi L, Marchand V, Dersh D, Gibbs JS, Ivanov IP, Fridlyand N, Couté Y, Diaz JJ, Qian SB, Staudt LM, Restifo NP, Yewdell JW. Ribosomal Proteins Regulate MHC Class I Peptide Generation for Immunosurveillance. Mol Cell. 2019 Jan 24.
10.1016/j.molcel.2018.12.020 ,
30712990 ,
HAL-02007743 Galvanin A, Ayadi L, Helm M, Motorin Y, Marchand V. Mapping and Quantification of tRNA 2'-O-Methylation by RiboMethSeq. Methods Mol Biol. 2019 ; 1870:273-295.
10.1007/978-1-4939-8808-2_21 ,
30539563 ,
HAL-01957102 Ayadi L, Galvanin A, Pichot F, Marchand V, Motorin Y. RNA ribose methylation (2'-O-methylation): Occurrence, biosynthesis and biological functions. Biochim Biophys Acta Gene Regul Mech. 2019 Mar.
10.1016/j.bbagrm.2018.11.009 ,
30572123 ,
HAL-01969343 Motorin Y, Marchand V. Detection and Analysis of RNA Ribose 2'-O-Methylations:Challenges and Solutions. Genes (Basel). 2018 Dec 18 ; 9(12).
10.3390/genes9120642 ,
30567409 ,
HAL-01969342 Marchand V, Ayadi L, Ernst FGM, Hertler J, Bourguignon-Igel V, Galvanin A, Kotter A, Helm M, Lafontaine DLJ, Motorin Y. AlkAniline-Seq: profiling of m7G and m3C RNA modifications at single nucleotide resolution. Angew Chem Int Ed Engl.2018 Oct 29.
10.1002/anie.201810946 ,
30370969 ,
HAL-01928435 Gachet S, El-Chaar T, Avran D, Genescà E, Catez F, Quentin S, Delord M, Thérizols G, Briot D, Meunier G, Hernandez L, Pla M, Smits WK, Buijs-Gladdines JGCAM, Van Loocke W, Menschaert G, André-Schmutz I, Taghon T, Van Vlierberghe P, Meijerink JP, Baruchel A, Dombret H, Clappier E, Diaz JJ, Gazin C, de The H, Sigaux F, Soulier J. Deletion 6q drives T-cell leukemia progression by ribosome modulation. Cancer Discov. 2018 Sep 28.
10.1158/2159-8290.CD-17-0831 ,
30266814 Fujikane R, Behm-Ansmant I, Tillault AS, Loegler C, Igel-Bourguignon V, Marguet E, Forterre P, Branlant C, Motorin Y, Charpentier B. Contribution of protein Gar1 to the RNA-guided and RNA-independent rRNA:Ψ-synthase activities of the archaeal Cbf5 protein. Sci Rep. 2018 Sep 14 ; 8(1):13815.
10.1038/s41598-018-32164-0 ,
30218085 ,
HAL-01881964 Khalil A, Medfai H, Poelvoorde P, Fayyad-Kazan M, Delporte C, Van Antwerpen P, El-Makhour Y, Biston P, Delrée P, Badran B, Vanhamme L, Boudjeltia KZ. Myeloperoxidase promotes tube formation, triggers ERK1/2 and Akt pathways and is expressed endogenously in endothelial cells. Arch Biochem Biophys. 2018 Sep 15 ; 654:55-69.
10.1016/j.abb.2018.07.011 ,
30016634
Formation CNRS Entreprises
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The EpiRNA-Seq Core Facility organizes the following training school (CNRS Formation Entreprises) : Epitranscriptomics: mapping and analysis of RNA modifications by Next-Generation Sequencing. This training school will be held from 17st to 19st May 2021 on the Core Facility in Nancy. For more details : https://cnrsformation.cnrs.fr/epitranscriptomics-mapping-and-analysis-of-rna-modifications-by-next-generation-sequencing?axe=140
Epitranscriptomics training school (COST Epitran)
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The NGS Core Facility in collaboration with Pr Iouri Motorine (UMR7365 IMoPA) is organizing a training school on Epitranscriptomics with the support of COST Epitran from 11-14 february 2019 in Nancy (France). This training is dedicated to young phD students or postdocs. The session in 2019 is closed. The next session will take place in February 2020, please send an email to the head of EpiRNA-Seq core facility. For more information : /sites/default/files/users/Projets/Diapositive1.png
