- Facility
Epitranscriptomics & Sequencing (EpiRNA-Seq)
- Next-Generation Sequencing, RNA, RNA modifications
The Epitranscriptomics and Sequencing (EpiRNA-Seq) Core Facility is built on the expertise of the UMR7365 IMoPA in the field of RNA molecular biology to promote its main collaborative services.
Located on the Brabois-Santé campus of the University of Lorraine in Nancy, it provides to the academic and industrial scientific community high-tech resources (Illumina next generation sequencers). It is part of the European COST EPITRAN network and regularly organises training activities.
The core facility has recognized technical and methodological know-how to contribute to various projects in Epitranscriptomics (RiboMethSeq, AlkAniline-Seq, HydraPsiSeq) to address various scientific questions such as dynamics of post-transcriptional modifications in RNAs.
The EpiRNA-Seq Core Facility operates mainly in collaborative mode (involvement of members of the core facility in the steps of your sequencing project from the conception of the experimental design to the statistical analysis of the data) but the delivery mode (mainly sequencing of libraries prepared by you without involvement of the platform) is also possible under certain conditions. The EpiRNA-Seq Core Facility has a differential pricing system (Université de Lorraine members, academics and industrialists).
The EpiRNA-Seq Core Facility is open to the academic and industrial scientific community at the local, national and international level.
The team is at your disposal to help you by bringing its technical and methodological know-how and to follow you from A to Z in your sequencing projects.
The EpiRNA-Seq Core Facility has a differential pricing system (Université de Lorraine members, academic and industrial).
Any new collaborator must first contact the head of the core facility using the "Request form" to define the best strategy to adopt for conducting his study, and obtain a quote. Any user will agree to accept the "terms and conditions". The guidelines to prepare and send samples to the Core Facility are given in "Sample sheet".
Keller P, Freund I, Marchand V, Bec G, Huang R, Motorin Y, Eigenbrod T, Dalpke A, Helm M. Double methylation of tRNA-U54 to 2'-O-methylthymidine (Tm) synergistically decreases immune response by Toll-like receptor 7. Nucleic Acids Res. 2018 Oct 12 ; 46(18):9764-9775.
10.1093/nar/gky644 , 
30102387 , 
HAL-01864193 Dal Magro C, Keller P, Kotter A, Werner S, Duarte V, Marchand V, Ignarski M, Freiwald A, Müller RU, Dieterich C, Motorin Y, Butter F, Atta M, Helm M. A Vastly Increased Chemical Variety of RNA Modifications Containing a Thioacetal Structure. Angew Chem Int Ed Engl. 2018 Jun 25 ; 57(26):7893-7897.
10.1002/anie.201713188 ,
29624844 ,
HAL-01801500 Aschenbrenner J, Werner S, Marchand V, Adam M, Motorin Y, Helm M, Marx A. Engineering of a DNA Polymerase for Direct m(6) A Sequencing. Angew Chem Int Ed Engl. 2018 Jan 8 ; 57(2):417-421.
10.1002/anie.201710209 ,
29115744 ,
HAL-01801461 Ayadi L, Motorin Y, Marchand V. Quantification of 2'-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMethSeq Protocol). Methods Mol Biol. 2018 ; 1649:29-48.
10.1007/978-1-4939-7213-5_2 ,
29130188 ,
HAL-01661952 Guéant JL, Chéry C, Oussalah A, Nadaf J, Coelho D, Josse T, Flayac J, Robert A, Koscinski I, Gastin I, Filhine-Tresarrieu P, Pupavac M, Brebner A, Watkins D, Pastinen T, Montpetit A, Hariri F, Tregouët D, Raby BA, Chung WK, Morange PE, Froese DS, Baumgartner MR, Benoist JF, Ficicioglu C, Marchand V, Motorin Y, Bonnemains C, Feillet F, Majewski J, Rosenblatt DS. APRDX1 mutant allele causes a MMACHC secondary epimutation in cblC patients. Nat Commun. 2018 Jan 4 ; 9(1):67.
10.1038/s41467-017-02306-5 ,
29302025 ,
HAL-01801484 Erales J, Marchand V, Panthu B, Gillot S, Belin S, Ghayad SE, Garcia M, Laforêts F, Marcel V, Baudin-Baillieu A, Bertin P, Couté Y, Adrait A, Meyer M, Therizols G, Yusupov M, Namy O, Ohlmann T, Motorin Y, Catez F, Diaz JJ. Evidence for rRNA 2'-O-methylation plasticity: Control of intrinsic translational capabilities of human ribosomes. Proc Natl Acad Sci U S A. 2017 Dec 5 ; 114(49):12934-12939.
10.1073/pnas.1707674114 ,
29158377 ,
HAL-01866982 Sharma S, Marchand V, Motorin Y, Lafontaine DLJ. Identification of sites of 2'-O-methylation vulnerability in human ribosomal RNAs by systematic mapping. Sci Rep. 2017 Sep 13 ; 7(1):11490.
10.1038/s41598-017-09734-9 ,
28904332 ,
HAL-01799319 Marchand V, Ayadi L, El Hajj A, Blanloeil-Oillo F, Helm M, Motorin Y. High-Throughput Mapping of 2'-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMethSeq Protocol). Methods Mol Biol. 2017 ; 1562:171-187.
10.1007/978-1-4939-6807-7_12 ,
28349461 ,
HAL-01799289 Marchand V, Pichot F, Thüring K, Ayadi L, Freund I, Dalpke A, Helm M, Motorin Y. Next-Generation Sequencing-Based RiboMethSeq Protocol for Analysis of tRNA 2'-O-Methylation. Biomolecules. 2017 Feb 9 ; 7(1).
10.3390/biom7010013 ,
28208788 ,
HAL-01799272
Formation CNRS Entreprises
( -> )
The EpiRNA-Seq Core Facility organizes the following training school (CNRS Formation Entreprises) : Epitranscriptomics: mapping and analysis of RNA modifications by Next-Generation Sequencing. This training school will be held from 17st to 19st May 2021 on the Core Facility in Nancy. For more details : https://cnrsformation.cnrs.fr/epitranscriptomics-mapping-and-analysis-of-rna-modifications-by-next-generation-sequencing?axe=140
Epitranscriptomics training school (COST Epitran)
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The NGS Core Facility in collaboration with Pr Iouri Motorine (UMR7365 IMoPA) is organizing a training school on Epitranscriptomics with the support of COST Epitran from 11-14 february 2019 in Nancy (France). This training is dedicated to young phD students or postdocs. The session in 2019 is closed. The next session will take place in February 2020, please send an email to the head of EpiRNA-Seq core facility. For more information : /sites/default/files/users/Projets/Diapositive1.png
