Iouri MOTORINE
Scientific Head
Professor, UL
contact
UMR7365 CNRS-Université de Lorraine
Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA)
Biopôle, Campus Brabois-Santé
9 Avenue de la Forêt de Haye, BP 20199
54505 VANDOEUVRE-LES-NANCY
Biography
Education
- 1999 : Habilitation to conduct research (HDR), University of Paris Sud, Orsay
- 1989 : phD Thesis in Chemistry, Institute of Biochemistry A.N. Bakh, Moscow
- 1985-1989 : phD, Evolutionary Biochemistry Lab, Institute of Biochemistry A.N. Bakh, Moscow
- 1985 : Master Thesis (DEA), Lomonossov University of Moscow, Evolutionary Biochemistry Lab, Institute of Biochemistry A.N. Bakh, Moscow
Experiences
- 2000-Present : Professor of Molecular Biology at the Université de Lorraine, Nancy, France. Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA) lab, UMR7365 CNRS-Université de Lorraine. Co-PI of the team "RNA-RNP Maturation-Structure-Fonction", axis "New technologies for RNA modifications detection"
- 1999-2000 : Associated Researcher, Maturation des ARN et Enzymologie Moléculaire laboratory, UMR 7567 UHP-CNRS, Nancy, France
- 1998-1999 : Associated Researcher, Enzymology laboratory, Gif-sur-Yvette, France
- 1994-1997 : Postdoc, Enzymology laboratory, Gif-sur-Yvette, France
- 1993-1994 : Postdoc, Enzymology laboratory, Gif-sur-Yvette, France
- 1991-1993 : Researcher, Institute of Biochemistry A.N. Bakh, Moscow
- 1992 : Postdoc, Enzymology laboratory, Gif-sur-Yvette, France
- 1989-1991 : Researcher, Institute of Biochemistry A.N. Bakh, Moscow
- 1985-1989 : Junior scientist, Institute of Biochemistry A.N. Bakh, Moscow
Responsabilities
- Director of UMS2008 IBSLor, CNRS-UL-INSERM
- Co-PI of team 1 "RNA-RNP, Structure-Fonction-Maturation" of IMoPA laboratory (UMR7365 CNRS-Université de Lorraine) and in charge of the axis "New technologies for RNA modifications detection"
- Member of management commitee of the COST action EPITRAN (2017-2021)
- Scientific Partner of ANR - PRC grant "RiboMet" (2021-2024)
- Coordinator of Regional Program FRCR "ViroMod" (2021-2024)
- Member of Editorial Board for journals Scientific Reports (Nature Group) et Genes
- Referee for different journals (Nature, Nature Biotechnology, Nucleic Acids Research, Scientific Reports, WIRES RNA, RNA Biology, RNA, PLoS One, BMC Biochemistry, International Journal of Molecular Sciences / Reactome website, Methods Elsevier, Genes)
- Expert for national and international funding agencies
research
Research
Topics
- From September 2000 on, analysis of RNA modifications
- Study and characterization of a new family of pseudouridine synthases
- Study and Characterization of a multifunctional RNA:pseudouridine-synthase responsible of pseudouridines in tRNA, U2 snRNA and 5S rRNA in yeast S. cerevisiae
- Study of RNA:m5C-methyltransferases
- In 2008-2009, collaboration project with the team of Dr Mark Helm at the University of Heildeberg (Germany)
- Use of chemicals for specific RNA labelling
- From 2011 on, in collaboration with Pr Mark Helm, development of high-throughput approaches to identify and characterize of RNA modifications
- Development of RT-seq (m1A)
- Development of RiboMethSeq (2'-O-Me)
- Development of AlkAnilineSeq (m3C and m7G)
- Development of HydraPsiSeq (Psi)
Key
Results
- High-throughput approaches to study RNA modifications
Ringeard M, Marchand V, Decroly E, Motorin Y, Bennasser Y. FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing. Nature. 2019 Jan 9. doi: 10.1038/s41586-018-0841-4. PMID:30626973.
Marchand V, Pichot F, Neybecker P, Ayadi L, Bourguignon-Igel V, Wacheul L, Lafontaine DLJ, Pinzano A, Helm M, Motorin Y. HydraPsiSeq: a method for systematic and quantitative mapping of pseudouridines in RNA. Nucleic Acids Res. 2020 Sep 25 : gkaa769. doi:10.3390/genes11080950
Marchand V, Ayadi L, Ernst FGM, Hertler J, Bourguignon-Igel V, Galvanin A, Kotter A, Helm M, Lafontaine DLJ, Motorin Y. AlkAniline-Seq: Profiling of m(7)G and m(3)C RNA Modifications at Single Nucleotide Resolution. Angew Chem Int Ed Engl. 2018 Dec 17 ; 57(51):16785-16790. doi: 10.1002/anie.201810946. PMID: 30370969.
Marchand V, Blanloeil-Oillo F, Helm M, Motorin Y. Illumina-based RiboMethSeq approach for mapping of 2'-O-Me residues in RNA. Nucleic Acids Res. 2016 Sep19 ; 44(16):e135. doi: 10.1093/nar/gkw547. PMID: 27302133.
- Characterization of m5C-methyltransferases
Bourgeois G, Ney M, Gaspar I, Aigueperse C, Schaefer M, Kellner S, Helm M, Motorin Y. Eukaryotic rRNA Modification by Yeast 5-Methylcytosine-Methyltransferases and Human Proliferation-Associated Antigen p120. PLoS One. 2015 Jul 21 ; 10(7):e0133321. doi: 10.1371/journal.pone.0133321. PMID: 26196125.
Motorin Y, Burhenne J, Teimer R, Koynov K, Willnow S, Weinhold E, Helm M. Expanding the chemical scope of RNA:methyltransferases to site-specific alkynylation of RNA for click labeling. Nucleic Acids Res. 2011 Mar ; 39(5):1943-52. doi: 10.1093/nar/gkq825. PMID:21037259.
- Characterization of pseudouridines-synthases
Behm-Ansmant I, Branlant C, Motorin Y. The Saccharomyces cerevisiae Pus2 protein encoded by YGL063w ORF is a mitochondrial tRNA:Psi27/28-synthase. RNA.2007 Oct ; 13(10):1641-7. PMID: 17684231.
Behm-Ansmant I, Massenet S, Immel F, Patton JR, Motorin Y, Branlant C. A previously unidentified activity of yeast and mouse RNA:pseudouridine synthases 1 (Pus1p) on tRNAs. RNA. 2006 Aug ; 12(8):1583-93. PMID:16804160.
Publications
