Facility
Epitranscriptomics & Sequencing (EpiRNA-Seq)
Ultrasonicateur M220 (Covaris)
The M220 Focused-ultrasonicator is based on the Covaris AFA technology.
Acoustic energy is transmitted at very short wavelengths (~3 mm) enabling the energy to be focused into your sample with a precise control of the hydrodynamic shearing forces. The process is isothermal, non contact, and quiet. Energy delivered is highly tunable, ranging from very gentle mixing to high-power tissue disruption, thus allowing a wide range of applications and making the M220 uniquely versatile.
Covaris AFA technology coupled with integrated temperature control eliminates operator induced variations, improves recoveries, and helps you achieve sensitive, standardized, and reproducible results.
This instrument makes it possible to shear DNA or chromatin for the preparation of libraries for high throughput sequencing.
The Covaris M200 model:
- uses a proven Covaris AFA fragmentation process.
- allows homogeneous and highly reproducible fragment distributions.
- includes fragmentation programs optimized to generate fragments of between 150 and 5000 bp in size.
- Request form60.36 KB
- Terms and conditions201.08 KB
This equipment is available for booking. If you want to use this equipment, please fill out the "Request form" to contact the core facility and obtain a quote. Any user will agree to accept the terms and conditions.
Fujikane R, Behm-Ansmant I, Tillault AS, Loegler C, Igel-Bourguignon V, Marguet E, Forterre P, Branlant C, Motorin Y, Charpentier B. Contribution of protein Gar1 to the RNA-guided and RNA-independent rRNA:Ψ-synthase activities of the archaeal Cbf5 protein. Sci Rep. 2018 Sep 14 ; 8(1):13815.
10.1038/s41598-018-32164-0 , 
30218085 , 
HAL-01881964
