- Facility
Epitranscriptomics & Sequencing (EpiRNA-Seq)
- Next-Generation Sequencing, RNA, RNA modifications
The Epitranscriptomics and Sequencing (EpiRNA-Seq) Core Facility is built on the expertise of the UMR7365 IMoPA in the field of RNA molecular biology to promote its main collaborative services.
Located on the Brabois-Santé campus of the University of Lorraine in Nancy, it provides to the academic and industrial scientific community high-tech resources (Illumina next generation sequencers). It is part of the European COST EPITRAN network and regularly organises training activities.
The core facility has recognized technical and methodological know-how to contribute to various projects in Epitranscriptomics (RiboMethSeq, AlkAniline-Seq, HydraPsiSeq) to address various scientific questions such as dynamics of post-transcriptional modifications in RNAs.
The EpiRNA-Seq Core Facility operates mainly in collaborative mode (involvement of members of the core facility in the steps of your sequencing project from the conception of the experimental design to the statistical analysis of the data) but the delivery mode (mainly sequencing of libraries prepared by you without involvement of the platform) is also possible under certain conditions. The EpiRNA-Seq Core Facility has a differential pricing system (Université de Lorraine members, academics and industrialists).
The EpiRNA-Seq Core Facility is open to the academic and industrial scientific community at the local, national and international level.
The team is at your disposal to help you by bringing its technical and methodological know-how and to follow you from A to Z in your sequencing projects.
The EpiRNA-Seq Core Facility has a differential pricing system (Université de Lorraine members, academic and industrial).
Any new collaborator must first contact the head of the core facility using the "Request form" to define the best strategy to adopt for conducting his study, and obtain a quote. Any user will agree to accept the "terms and conditions". The guidelines to prepare and send samples to the Core Facility are given in "Sample sheet".
- Kilz LM, Zimmermann S, Marchand V, Bourguignon V, Sudol C, Brégeon D, Hamdane D, Motorin Y, Helm M. Differential redox sensitivity of tRNA dihydrouridylation. Nucleic Acids Res. 2024 Nov 27;52(21):12784-12797.
10.1093/nar/gkae964 , 
39460624 , 
HAL-04911631 - Henkel M, Fillbrunn A, Marchand V, Raghunathan G, Berthold MR, Motorin Y, Marx A. A DNA Polymerase Variant Senses the Epigenetic Marker 5-Methylcytosine by Increased Misincorporation. Angew Chem Int Ed Engl. 2024 Nov 25;63(48):e202413304.
10.1002/anie.202413304 ,
39449390 ,
HAL-04939883
- Dominique C, Maiga NK, Méndez-Godoy A, Pillet B, Hamze H, Léger-Silvestre I, Henry Y, Marchand V, Gomes Neto V, Dez C, Motorin Y, Kressler D, Gadal O, Henras AK, Albert B. The dual life of disordered lysine-rich domains of snoRNPs in rRNA modification and nucleolar compaction. Nat Commun. 2024 Oct 31;15(1):9415.
10.1038/s41467-024-53805-1 ,
39482307 ,
HAL-04797120
- Schartel L, Jann C, Wierczeiko A, Butto T, Mündnich S, Marchand V, Motorin Y, Helm M, Gerber S, Lemke EA. Selective RNA pseudouridinylation in situ by circular gRNAs in designer organelles. Nat Commun. 2024 Oct 24;15(1):9177.
10.1038/s41467-024-53403-1 ,
39448590 ,
HAL-04939840
- Kristen M, Lander M, Kilz LM, Gleue L, Jörg M, Bregeon D, Hamdane D, Marchand V, Motorin Y, Friedland K, Helm M. DORQ-seq: high-throughput quantification of femtomol tRNA pools by combination of cDNA hybridization and Deep sequencing. Nucleic Acids Res. 2024 Oct 14;52(18):e89.
10.1093/nar/gkae765 ,
39258547
- Rahman ML, Bonnard AA, Wang F, Ruaud L, Guimiot F, Li Y, Defer I, Wang Y, Marchand V, Motorin Y, Yao B, Drunat S, Ghalei H. New ZNHIT3 Variants Disrupting snoRNP Assembly Cause Prenatal PEHO Syndrome with Isolated Hydrops. medRxiv [Preprint]. 2024 Aug 26:2024.08.26.24312490.
10.1101/2024.08.26.24312490 ,
39252897
- Toubdji S, Thullier Q, Kilz LM, Marchand V, Yuan Y, Sudol C, Goyenvalle C, Jean-Jean O, Rose S, Douthwaite S, Hardy L, Baharoglu Z, de Crécy-Lagard V, Helm M, Motorin Y, Hamdane D, Brégeon D. Exploring a unique class of flavoenzymes: Identification and biochemical characterization of ribosomal RNA dihydrouridine synthase. Proc Natl Acad Sci U S A. 2024 Aug 6;121(32):e2401981121.
10.1073/pnas.2401981121 ,
39078675 ,
HAL-04734945
- Sudol C, Kilz LM, Marchand V, Thullier Q, Guérineau V, Goyenvalle C, Faivre B, Toubdji S, Lombard M, Jean-Jean O, de Crécy-Lagard V, Helm M, Motorin Y, Brégeon D, Hamdane D. Functional redundancy in tRNA dihydrouridylation. Nucleic Acids Res. 2024 Jun 10;52(10):5880-5894.
10.1093/nar/gkae325 ,
38682613 ,
HAL-04614743
- Van den Akker GGH, Chabronova A, Housmans BAC, van der Vloet L, Surtel DAM, Cremers A, Marchand V, Motorin Y, Caron MMJ, Peffers MJ, Welting TJM. TGF-β2 Induces Ribosome Activity, Alters Ribosome Composition and Inhibits IRES-Mediated Translation in Chondrocytes. Int J Mol Sci. 2024 May 5;25(9):5031.
10.3390/ijms25095031 ,
38732249 ,
HAL-04934350
- Sklias A, Cruciani S, Marchand V, Spagnuolo M, Lavergne G, Bourguignon V, Brambilla A, Dreos R, Marygold SJ, Novoa EM, Motorin Y, Roignant JY. Comprehensive map of ribosomal 2'-O-methylation and C/D box snoRNAs in Drosophila melanogaster. Nucleic Acids Res. 2024 Apr 12;52(6):2848-2864.
10.1093/nar/gkae139 ,
38416577 ,
HAL-04934064
Formation CNRS Entreprises
( -> )
The EpiRNA-Seq Core Facility organizes the following training school (CNRS Formation Entreprises) : Epitranscriptomics: mapping and analysis of RNA modifications by Next-Generation Sequencing. This training school will be held from 17st to 19st May 2021 on the Core Facility in Nancy. For more details : https://cnrsformation.cnrs.fr/epitranscriptomics-mapping-and-analysis-of-rna-modifications-by-next-generation-sequencing?axe=140
Epitranscriptomics training school (COST Epitran)
( -> )
The NGS Core Facility in collaboration with Pr Iouri Motorine (UMR7365 IMoPA) is organizing a training school on Epitranscriptomics with the support of COST Epitran from 11-14 february 2019 in Nancy (France). This training is dedicated to young phD students or postdocs. The session in 2019 is closed. The next session will take place in February 2020, please send an email to the head of EpiRNA-Seq core facility. For more information : /sites/default/files/users/Projets/Diapositive1.png
