"EpiRNA-Seq" (Epitranscriptomics and RNA Sequencing) is the new name of the Next-Generation Sequencing Core Facility (NGS). It will shed light on the flagship activities of the core facility in the field of epitranscriptomics. Spotlight for this unique specialization in France and Europe ...

What is epitranscriptome and why is it so important?

RNAs are modified after their transcription, so in addition to the canonical bases of RNA (A, C, G, U), additions of chemical groups or creation of new bases will take place mainly by the action of enzymes or ribonucleoprotein particles. Currently, there are more than 120 modifications and some of them participate in the fine regulation of gene expression and play a role in the efficiency and fidelity of translation. They could even serve as biomarkers of diseases (cancer, rare diseases, ...), aging or in immunity. Their mapping by high throughput sequencing has become essential for a better understanding of these biological mechanisms.

The EpiRNA-Seq Core Facility has a unique experience and expertise with RNA and is developing new techniques in the field of epitranscriptomics to be able to share them with the scientific community.

The expertise of the EpiRNA-Seq core facility is based on a unique and recognized expertise in RNA manipulation.

The Core Faciltiy has developed its own methodologies for mapping 2'-O-methylated residues (Nm), m⁷G and m³C.

To support you in all your projects, it proposes to take charge of different stages of your projects starting with the extraction of RNAs, their treatments with chemical reagents, and the preparation of dedicated sequencing libraries. These modifications are mainly studied in abundant RNA (ribosomal RNA or transfer RNA) but can also be studied at the level of the transcriptome (mRNA and lncRNA). Its combination with advanced technologies (Illumina sequencing platform), and exclusive bioinformatics pipelines regularly adapted to projects and constantly improved, guarantee you high-performance and high-quality data.

In addition, the EpiRNA-Seq Core Facility continues to develop new and innovative research approaches to map other types of modified nucleotides to make them available to the scientific community as quickly as possible.

Despite the specialization of the EpiRNA-Seq Core Facility, we also provide transcriptomics (RNA-seq and miRNA-seq) services.

For additional information, please contact the EpiRNA-Seq Core Facility : ibslor-contact-ngs [chizindikiro] univ-lorraine [mfundo] fr