Autoflex speed IV

Specifications

Specificities :

  • It is a mass spectrometer with MALDI ionization and linear and reflectron TOF analyzers including CID fragmentation chamber.
  • The flight tube of the Autoflex IV in maintained under a vaccum in the 10-7 bar order to allow mass measurement precisions in the 10 ppm range.
  • Most analyses performed on our mass spectrometer are fully automated and autonomous.
  • The ionization source of our Autoflex IV is cleaned up manually on a weekly basis in order to maintain optimal sensitivity.
Type
MALDI-TOF/TOF mass spectrometer
Reference
Autoflex speed IV (Bruker)
pf-proteomique

Access and Fees

  • This equipment is not available for booking. Please contact the Core Facility manager.

Publications

  • Henrionnet C, Gillet P, Mainard D, Vincourt JB, Pinzano A. Label-free relative quantification of secreted proteins as a non-invasive method for the quality control of chondrogenesis in bioengineered substitutes for cartilage repair. J Tissue Eng Regen Med. 2018 Mar ; 12(3):e1757-e1766.

    logo DOI - Digital Object Identifier 10.1002/term.2454 , Logo PMID - PubMed 28485490 , logo HAL - Archives Ouvertes HAL-01745215
  • Belcastro E, Wu W, Fries-Raeth I, Corti A, Pompella A, Leroy P, Lartaud I, Gaucher C. Oxidative stress enhances and modulates protein S-nitrosation in smooth muscle cells exposed to S-nitrosoglutathione. Nitric Oxide. 2017 Sep 30 ; 69:10-21.

    logo DOI - Digital Object Identifier 10.1016/j.niox.2017.07.004 , Logo PMID - PubMed 28743484
  • Luo M, Boudier A, Pallotta A, Maincent P, Vincourt JB, Leroy P. Albumin as a carrier for NO delivery: preparation, physicochemical characterization, and interaction with gold nanoparticles. Drug Dev Ind Pharm. 2016 Dec ; 42(12):1928-1937.

    logo DOI - Digital Object Identifier 10.1080/03639045.2016.1182546 , Logo PMID - PubMed 27126574 , logo HAL - Archives Ouvertes HAL-01380405
  • Riffault M, Moulin D, Grossin L, Mainard D, Magdalou J, Vincourt JB. Label-free relative quantification applied to LC-MALDI acquisition for rapid analysis of chondrocyte secretion modulation. J Proteomics. 2015 Jan 30 ; 114:263-73.

    logo DOI - Digital Object Identifier 10.1016/j.jprot.2014.10.026 , Logo PMID - PubMed 25464362 , logo HAL - Archives Ouvertes HAL-01451913