Label-free relative quantification by LC-MALDI
This service requires experimental replicates within each biological group to be considered in comparative analysis.
This service includes :
- Chemical and enzymatic treatment of samples, being either polyacrylamide gel pieces or water-based solutions (with restrictions regarding buffer contents), for exhaustive identification of protein content.
- NanoHPLC fractionation.
- MALDI MS and MS/MS analysis.
- Database interrogation.
- Analytical report.
- Request form307.5 KB
- Terms and conditions358.47 KB
If you are interested in this service, please contact the Core Facility manager to define the best strategy to adopt for conducting your study, and obtain a quote. Any user will agree to accept the terms and conditions.
Henrionnet C, Gillet P, Mainard D, Vincourt JB, Pinzano A. Label-free relative quantification of secreted proteins as a non-invasive method for the quality control of chondrogenesis in bioengineered substitutes for cartilage repair. J Tissue Eng Regen Med. 2018 Mar ; 12(3):e1757-e1766.
10.1002/term.2454 , 
28485490 , 
HAL-01745215 Riffault M, Moulin D, Grossin L, Mainard D, Magdalou J, Vincourt JB. Label-free relative quantification applied to LC-MALDI acquisition for rapid analysis of chondrocyte secretion modulation. J Proteomics. 2015 Jan 30 ; 114:263-73.
10.1016/j.jprot.2014.10.026 ,
25464362 ,
HAL-01451913
