Facility
Epitranscriptomics & Sequencing (EpiRNA-Seq)
Sample Preparation / RNA extraction and quality control
RNA extraction of a tissue or cells
The RNA extraction step from a tissue or cells from cultures or biological samples is an essential step in the proper conduct of transcriptomic and epitranscriptomic analyses and the interpretation of the results.
The core facility can perform the RNA extraction step from pellets of:
- Cell cultures (monolayer or suspension)
- Bacteria, Yeast, ...
and continue with an enrichment step of certain RNAs (small RNA, tRNA, mRNA, etc.).
The quality of the extracted RNAs is highly dependent on the conditions under which the samples were prepared, collected and stored. Once the RNA extraction step has been carried out, it is necessary to subject the samples to a qualitative and quantitative control:
- The amount of extracted RNAs is determined by spectrophotometry (Nanodrop One ™ spectrophotometer, Ozyme).
- The quality of extracted RNA is assessed by capillary electrophoresis using Agilent® Bioanalyzer 2100. The level of RNA quality is associated with a RIN value determined by the device: the closer this value is to 10, the better the RNA quality is.
- Request form160.17 KB
- Terms and conditions201.08 KB
- Sample sheet186.79 KB
- Roadmap Bioanalyzer127.95 KB
This service is optional and can be included (if needed) for your projects (transcriptomics and epitranscriptomics). Please contact the head of the NGS core facility for more details.
Galvanin A, Ayadi L, Helm M, Motorin Y, Marchand V. Mapping and Quantification of tRNA 2'-O-Methylation by RiboMethSeq. Methods Mol Biol. 2019 ; 1870:273-295.
10.1007/978-1-4939-8808-2_21 , 
30539563 , 
HAL-01957102 Marchand V, Pichot F, Thüring K, Ayadi L, Freund I, Dalpke A, Helm M, Motorin Y. Next-Generation Sequencing-Based RiboMethSeq Protocol for Analysis of tRNA 2'-O-Methylation. Biomolecules. 2017 Feb 9 ; 7(1).
10.3390/biom7010013 ,
28208788 ,
HAL-01799272
