- Facility
Flow Cytometry
, 
- Cell cycle, Extracellular vesicles, Phenotyping, Sorting, Apoptosis, Mitochondrial respiration and glycolysis, Real-time imaging
The Flow Cytometry and Cell Biology Platform is an integral part of the Shared Engineering Platforms for Biology and Health Service (IBSLor) in Lorraine.
Located on the Brabois Health Campus of the University of Lorraine in Nancy, the platform provides state-of-the-art equipment for a wide range of cell analysis and cell sorting applications. It offers three types of services within an appropriate environment designed to support the development of scientific research projects:
- Flow Cytometry Analysis :
- BD Biosciences FACSCelesta™ Flow Cytometer: 4 lasers, 16-color analysis. Training, instrument access, and analytical services.
- BD Biosciences FACSSymphony™ A3 Flow Cytometer: 5 lasers, 28-color analysis. Training, instrument access, and analytical services.
- Cell Sorting
- Beckman Coulter Astrios Cell Sorter: 3 lasers, 14-color analysis. Cell sorting performed by platform staff.
- Cellular Metabolism Analysis
- Agilent Seahorse XFp Analyzer: Training and instrument access.
- Real-Time Imaging
- Cytation™ 10 Imaging Reader coupled with BioSpa™: Training, instrument access, and imaging services.
The platform provides research teams with expertise and technical support to advance their scientific projects. User-oriented expertise covers a broad range of applications, including cell cycle and apoptosis analysis, rare cell sorting, and multiparametric cell sorting. The platform also benefits from recognized technical expertise in the detection and standardization of circulating human microparticle analysis. Its objective is to meet all flow cytometry and related laboratory research needs while remaining open to the entire academic scientific community (CNRS, INSERM, University of Lorraine, etc.) as well as industrial partners.
Our missions are to adapt the methodological developments of the cytometry Core Facility in relation with users' projects, to propose activities of transfer of knowledge of the domain to scientists, students and staff in training courses. We maintain a technology watch associated with the increase in the competence of the service offered.
The staff works to ensure the good functioning of the platform:
- Organization of the activity (reservation planning, data storage).
- Training / user support.
- Project monitoring and analysis.
- Laintenance and management of hardware and equipment.
Since 2020, the platform has been awarded the INFRA+ label by Lorraine Université d'Excellence (LUE).
- Flow Cytometry Analysis :
- Prices123.19 KBRequest form - Flow cytometry equipment100.99 KBRequest form for availability - Flow cytometry equipment104.69 KBRequest form - Astrios195.72 KBTerms and conditions890.62 KB
The Core Facility is open to the academic and industrial scientific communities at local, national, and international levels.
Our team provides technical and methodological expertise and supports users throughout every stage of their cytometry projects.
The Core Facility operates a differentiated pricing policy for members, academic users, and industrial partners.
New users are encouraged to contact the Core Facility Manager through the request form to discuss their project, define the most appropriate experimental strategy, and obtain a quotation.
All users are required to accept the Facility's terms and conditions before accessing its services.
To book an equipment, click here.
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HAL-03430881 Guéant JL, Guéant-Rodriguez RM, Fromonot J, Oussalah A, Louis H, Chery C, Gette M, Gleye S, Callet J, Raso J, Blanchecotte F, Lacolley P, Guieu R, Regnault V. Elastase and exacerbation of neutrophil innate immunity are involved in multi-visceral manifestations of COVID-19. Allergy. 2021 Jun;76(6):1846-1858.
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33484168 Ferhat M, Hablot J, Taieb M, Salem F, Netter P, Peyrin-Biroulet L, Jouzeau JY, Moulin D. Lack of protective effect of CCR3 blockade during experimental colitis may be related to CCR3 expression by colonic Tregs. Clin Transl Med. 2021 Jun;11(6):e455.
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34185413 Tran L, Pannier B, Lacolley P, Serrato T, Benetos A, London GM, Bézie Y, Regnault V. A case-control study indicates that coagulation imbalance is associated with arteriosclerosis and markers of endothelial dysfunction in kidney failure. Kidney Int. 2021 May;99(5):1162-1172.
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Flow Cytometry Training - Autonomy on Celesta and Symphony
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The Flow Cytometry Core Facility offers two training sessions (February and September-October) "Cytometry-Celesta and Symphony".
Program of a training course :
Theoretical (2h):
- Theoretical foundations of flow cytometry. Principles of operation.
- Reminder of some immunotechnology concepts.
- Multiparametric analysis: interest and associated constraints.
Practical (4h):
- Take charge of the instrument. Conditions of use. Discovery of the acquisition software.
- Creation of protocols and protocol suites.
- Instrument settings. Introduction of a multicolour analysis.
- Exploitation of the reprocessing capabilities of the acquisition software.
Trieur Astrios & Cytomètre Symphony :
Service Mutualisé de Plateformes
Biopôle, Campus Brabois-Santé
9 Avenue de la Forêt de Haye, BP 20199
54505 VANDOEUVRE-LES-NANCYCytomètre Celesta & Cytation & Seahorse :
Service Mutualisé de Plateformes - UMR1116 DCAC
Bâtiment D, 1er étage, Campus Brabois-Santé
9 Avenue de la Forêt de Haye, BP 20199
54505 VANDOEUVRE-LES-NANCY
