Facility
Biophysics & Structural Biology (B2S)
NMR
The NMR spectrometer is equipped with a 1H, 13C and 15N triple resonance TCI cryoprobe with Z-axis static field gradient and active shielding. The cryoprobe is cooled on the 1H and 13C nuclei, thus allowing a sensitivity gain that can reach a factor of 4 to 8 compared to a conventional probe. It can operate in the temperature range from 0 to + 60 ° C.
This device is suitable for the study of the 3D structure and dynamics of proteins, nucleic acids and protein / ligand interactions (antigen / antibody, enzyme / substrate, etc.), but also for the analysis of biological fluids, synthetic organic molecules, chemical polymers, etc.
The NMR spectrometer is equipped with a Linux PC workstation, with TopSpin 3.2 software.
Several services are available: Characterization of proteins, peptides, RNA and organic molecules in solution; Characterization of receptor-ligand interactions; Quality control of molecule in aqueous or organic solution ...
- Request form90.91 KB
- Terms and conditions319.06 KB
Analyzes can be performed in service mode or device provision mode in function of the expertise of the user. Contact the person in charge using the Request form for any information.
- Dos Santos Morais R, Santo PE, Ley M, Schelcher C, Abel Y, Plassart L, Deslignière E, Chagot ME, Quinternet M, Paiva ACF, Hessmann S, Morellet N, M F Sousa P, Vandermoere F, Bertrand E, Charpentier B, Bandeiras TM, Plisson-Chastang C, Verheggen C, Cianférani S, Manival X. Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases. J Mol Biol. 2022 Jul 25.
10.1016/j.jmb.2022.167760 , 
35901867 , 
HAL-03745381 Chagot ME, Boutilliat A, Kriznik A, Quinternet M. Structural Analysis of the Plasmodial Proteins ZNHIT3 and NUFIP1 Provides Insights into the Selectivity of a Conserved Interaction. Biochemistry. 2022 Mar 22.
10.1021/acs.biochem.1c00792 ,
35315277 ,
HAL-03617333 - Abel Y, Charron C, Virciglio C, Bourguignon-Igel V, Quinternet M, Chagot ME, Robert MC, Verheggen C, Branlant C, Bertrand E, Manival X, Charpentier B, Rederstorff M. The interaction between RPAP3 and TRBP reveals a possible involvement of the HSP90/R2TP chaperone complex in the regulation of miRNA activity. Nucleic Acids Res. 2022 Feb 28;50(4):2172-2189.
10.1093/nar/gkac086 ,
35150569 ,
HAL-03583324
Motorin Y, Quinternet M, Rhalloussi W, Marchand V. Constitutive and variable 2'-O-methylation (Nm) in human ribosomal RNA. RNA Biol. 2021 Sep 10:1-10.
10.1080/15476286.2021.1974750 ,
34503375 ,
HAL-03341858 - Bragantini B, Charron C, Bourguet M, Paul A, Tiotiu D, Rothé B, Marty H, Terral G, Hessmann S, Decourty L, Chagot ME, Strub JM, Massenet S, Bertrand E, Quinternet M, Saveanu C, Cianférani S, Labialle S, Manival X, Charpentier B. The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity. Nat Commun. 2021 Mar 25;12(1):1859.
10.1038/s41467-021-22077-4 ,
33767140 ,
HAL-03181046
Dermouche S, Chagot ME, Manival X, Quinternet M. Optimizing the First TPR Domain of the Human SPAG1 Protein Provides Insight into the HSP70 and HSP90 Binding Properties. Biochemistry. 2021 Mar 19.
10.1021/acs.biochem.1c00052 ,
33739091 ,
HAL-03175986 - Chagot ME, Quinternet M, Manival X, Lebars I. Application of NMR Spectroscopy to Determine the 3D Structure of Small Non-Coding RNAs. Methods Mol Biol. 2021;2300:251-266.
10.1007/978-1-0716-1386-3_19 ,
33792884 ,
HAL-03203667
Chagot ME, Quinternet M, Jacquemin C, Manival X, Gardiennet C. Box C/D snoRNPs: solid-state NMR fingerprint of an early-stage 50 kDa assembly intermediate. Biomol NMR Assign. 2020 Feb 6.
10.1007/s12104-020-09933-y ,
32030621 ,
HAL-02480749 - Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ. Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase. J Struct Biol. 2020 Jul 25.
10.1016/j.jsb.2020.107581 ,
32717326
Chagot ME, Dos Santos Morais R, Dermouche S, Lefebvre D, Manival X, Chipot C, Dehez F, Quinternet M. Binding properties of the quaternary assembly protein SPAG1. Biochem J. 2019 May 22.
10.1042/BCJ20190198 ,
31118266 ,
HAL-02140524 Chagot ME, Quinternet M, Rothé B, Charpentier B, Coutant J, Manival X, Lebars I. The yeast C/D box snoRNA U14 adopts a "weak" K-turn like conformation recognized by the Snu13 core protein in solution. Biochimie. 2019 Mar 23.
10.1016/j.biochi.2019.03.014 ,
30914254 ,
HAL-02082477 Bragantini B, Rouillon C, Charpentier B, Manival X, Quinternet M. NMR assignment and solution structure of the external DII domain of the yeast Rvb2 protein. Biomol NMR Assign. 2018 Oct ; 12(2):243-247.
10.1007/s12104-018-9816-5 ,
29569106 ,
HAL-01882005 Maurizy C, Quinternet M, Abel Y, Verheggen C, Santo PE, Bourguet M, Paiva A, Bragantini B, Chagot ME, Robert MC, Abeza C, Fabre P, Fort P, Vandermoere F,Sousa P Rain JC, Charpentier B, Cianférani S, Bandeiras TM, Pradet-Balade B, Manival X, Bertrand E. The RPAP3-Cterminal domain identifies R2TP-like quaternary chaperones. Nat Commun. 2018 May 29 ; 9(1):2093.
10.1038/s41467-018-04431-1 ,
29844425 ,
HAL-01872981 Henri J, Chagot ME, Bourguet M, Abel Y, Terral G, Maurizy C, Aigueperse C, Georgescauld F, Vandermoere F, Saint-Fort R, Behm-Ansmant I, Charpentier B, Pradet-Balade B, Verheggen C, Bertrand E, Meyer P, Cianférani S, Manival X, Quinternet M. Deep Structural Analysis of RPAP3 and PIH1D1, Two Components of the HSP90 Co-chaperone R2TP Complex. Structure. 2018 Sep 4 ; 26(9):1196-1209.
10.1016/j.str.2018.06.002 ,
30033218 ,
HAL-01919243 Kriznik A, Yéléhé-Okouma M, Lec JC, Groshenry G, Le Cordier H, Charron C, Quinternet M, Mazon H, Talfournier F, Boschi-Muller S, Jouzeau JY, Reboul P. CRDSAT Generated by pCARGHO: A New Efficient Lectin-Based Affinity Tag Method for Safe, Simple, and Low-Cost Protein Purification. Biotechnol J. 2018 Oct 8:e1800214.
10.1002/biot.201800214 ,
30298550 ,
HAL-01892441 Dorival J, Risser F, Jacob C, Collin S, Dräger G, Paris C, Chagot B, Kirschning A, Gruez A, Weissman KJ. Insights into a dual function amide oxidase/macrocyclase from lankacidin biosynthesis. Nat Commun. 2018 Sep 28 ; 9(1):3998.
10.1038/s41467-018-06323-w ,
30266997 ,
HAL-01980537
