Facility
Biophysics & Structural Biology (B2S)
Fast kinetics
The service offers a wide range of high performance equipments: two stopped-flow applied photophysics SX19 equipped with absorbance and fluorescence detectors; a stopped-flow applied photophysics SX20 equipped with CD and fluorescence detector; a Kin Tek RQF-3 quench-flow device with analysis of the mixture by UHPLC chromatography. This service makes it possible to measure very fast reaction kinetics, a crucial step in the fine understanding of enzymatic mechanisms.
- Request form90.91 KB
- Terms and conditions319.06 KB
Any new collaborator must contact the person in charge using the Request form to define the best strategy to adopt. Any user will accept the terms and conditions for use.
Lec J.C, Boutserin S, Mazon H, Mulliert G, Boschi-Muller S, Talfournier F. Unraveling the Mechanism of Cysteine Persulfide Formation Catalyzed by 3-Mercaptopyruvate Sulfurtransferases. ACS Catal. 2018 ; 8(3):2049–2059.
10.1021/acscatal.7b02432 , 
HAL-01715178 Rahuel-Clermont S, Bchini R, Barbe S, Boutserin S, André I, Talfournier F. Enzyme Active Site Loop Revealed as a Gatekeeper for Cofactor Flip by Targeted Molecular Dynamics Simulations and FRET-Based Kinetics. ACS Catal. 2019 ; 9 ; 1337−1346.
10.1021/acscatal.8b03951 ,
HAL-02022828
