Current
Research

High Throughput Methods to analyze RNA modifications (Epitranscriptomics) : Developments of new methods combining RNA chemistry, Molecular Biology and Next Generation Sequencing to identify RNA modifications (RiboMethSeq, AlkAniline-Seq, HydraPsiSeq, ...)

Key
Results

• High Throughput Methods to analyze RNA modifications (Epitranscriptomics)
  • Marchand V, Pichot F, Neybecker P, Ayadi L, Bourguignon-Igel V, Wacheul L, Lafontaine DLJ, Pinzano A, Helm M, Motorin Y. HydraPsiSeq: a method for systematic and quantitative mapping of pseudouridines in RNA. Nucleic Acids Res. 2020 Sep 25 : gkaa769. doi:10.3390/genes11080950.
  • Ringeard M, Marchand V, Decroly E, Motorin Y, Bennasser Y. FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing. Nature. 2019 Jan 9. doi:10.1038/s41586-018-0841-4.
  • Marchand V, Ayadi L, Ernst FGM, Hertler J, Bourguignon-Igel V, Galvanin A, Kotter A, Helm M, Lafontaine DLJ, Motorin Y. AlkAniline-Seq: Profiling of m(7)G and m(3)C RNA Modifications at Single Nucleotide Resolution. Angew Chem Int Ed Engl. 2018 Dec 17;57(51):16785-16790. doi:10.1002/anie.201810946.
  • Marchand V, Blanloeil-Oillo F, Helm M, Motorin Y. Illumina-based RiboMethSeq approach for mapping of 2'-O-Me residues in RNA. Nucleic Acids Res. 2016 Sep19;44(16):e135. doi: 10.1093/nar/gkw547.

• Molecular mechanisms implicated in Drosophila oskar mRNA localization and its translational control
  • Ghosh S, Obrdlik A, Marchand V, Ephrussi A. The EJC binding and dissociating activity of PYM is regulated in Drosophila. PLoS Genet. 2014 Jun 26;10(6):e1004455. doi:10.1371/journal.pgen.1004455.
  • Ghosh S*, Marchand V*, Gáspár I, Ephrussi A. Control of RNP motility and localization by a splicing-dependent structure in oskar mRNA. Nat Struct Mol Biol. 2012 Mar 18;19(4):441-9. doi:10.1038/nsmb.2257. *=co-first authors
  • Marchand V, Gaspar I, Ephrussi A. An intracellular transmission control protocol: assembly and transport of ribonucleoprotein complexes. Curr Opin Cell Biol. 2012 Apr;24(2):202-10. doi:10.1016/j.ceb.2011.12.014. Review.

• Molecular mechanisms implicated in alternative splicing of 2 viral RNAs : Rous Sarcoma virus (RSV) and HIV-1
  • Marchand V*, Santerre M*, Aigueperse C, Fouillen L, Saliou JM, Van Dorsselaer A, Sanglier-Cianférani S, Branlant C, Motorin Y. Identification of protein partners of the human immunodeficiency virus 1 tat/rev exon 3 leads to the discovery of a new HIV-1 splicing regulator, protein hnRNP K. RNA Biol. 2011 Mar-Apr;8(2):325-42. PMID: 21368586. *=co-first authors
  • Bar A, Marchand V, Khoury G, Dreumont N, Mougin A, Robas N, Stévenin J, Visvikis A, Branlant C. Structural and functional analysis of the Rous Sarcoma virus negative regulator of splicing and demonstration of its activation by the 9G8 SR protein. Nucleic Acids Res. 2011 Apr;39(8):3388-403. doi:10.1093/nar/gkq1114.
  • Marchand V, Méreau A, Jacquenet S, Thomas D, Mougin A, Gattoni R, Stévenin J, Branlant C. A Janus splicing regulatory element modulates HIV-1 tat and rev mRNA production by coordination of hnRNP A1 cooperative binding. J Mol Biol. 2002 Nov 1;323(4):629-52. PMID: 12419255.

Publications